High pure pcr product purification kit roche manual

The amplifications are gel controlled (2% agarose gel in 1X TBE) and rearrangement-positive PCR products are purified (High Pure PCR Product Purification Kit Roche) and sequenced on both strands (forward and reverse) according to the manual of the Applied Biosystem (Waltham, MA, USA) “Big dye Terminator v Cycle Sequencing kit”. High Pure PCR Product Purification Kit 20 Roche Applied Science has a wide array of manual nucleic acid prepara-tion products that are specifically designed to prepare DNA or RNA templates from a particular source or for certain applications (see “Overview” on pages File Size: KB. The High Pure RNA Isolation Kit isolates total RNA from cultured cells, free of any contaminating DNA. Other sample materials, such as whole blood, yeast, and bacteria require a pre-lysis treatment. Quickly process multiple samples. A single sample preparation is completed in 25 minutes, and multiple samples can be prepared in 45 minutes.

For purification of up to 10 μg PCR products, bp to 10 kb. The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products bp. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of μl. 2. How to Use this Product y Version: 26 High Pure PCR Template Preparation Kit 8 www.doorway.ru Sample Lysis and DNA Binding Protocol for the isolation of nucleic acids from: • μl mammalian blood • μl buffy coat • - 10 6 cultured mammalian cells Before starting the purification reaction, warm up the Elution Buffer to. www.doorway.ru For life science research only. Not for use in diagnostic procedures. R High Pure PCR Product Purification Kit y Version: 17 Content version: April For purification of PCR reaction products Cat. No. 11 1 kit up to 50 purifications Cat. No. 11 1 kit up to purifications Store the kit at +

8 www.doorway.ru 2. How to Use this Product y Version: 04 High Pure FFPET DNA Isolation Kit Deparaffinization The following steps describe the deparaffinization procedure for a single maximum 10 μm thick section of formalin-fixed, paraffin-embedded tissue in a ml reaction tube. 2. How to Use this Product y Version: 26 High Pure PCR Template Preparation Kit 8 www.doorway.ru Sample Lysis and DNA Binding Protocol for the isolation of nucleic acids from: • μl mammalian blood • μl buffy coat • - 10 6 cultured mammalian cells Before starting the purification reaction, warm up the Elution Buffer to. High Pure PCR Template Preparation Kit Rapidly purify genomic DNA for diverse applications Maximize flexibility. Use a single versatile kit to purify DNA from a broad range of sample materials. Facilitate long template applications. Efficiently purify high molecular weight DNA ( kb) (Figure 1). Improve assay results.